J.K. Simmons J. Jonah Jameson (voice)
Oscar Isaac Miguel O'Hara (voice)
Andrew Garfield Peter Parker / Spider-Man (archive footage) (uncredited)
Hailee Steinfeld Gwen Stacy (voice)
Denis Leary George Stacy (archive footage) (uncredited)
Tobey Maguire Peter Parker / Spider-Man (archive footage) (uncredited)
Kathryn Hahn Doc Ock (voice)
Andy Samberg Ben Reilly (voice)
Jason Schwartzman Spot (voice)
Daniel Kaluuya Hobie Brown (voice)
Mahershala Ali Uncle Aaron (voice)
Jake Johnson Peter B. Parker (voice)
Greta Lee LYLA (voice)
Shea Whigham George Stacy (voice)
Jack Quaid Peter Parker (voice)
Rachel Dratch Ms. Weber (voice)
Brian Michael Bendis Executive Producer
Donald Glover Aaron Davis
Issa Rae Jessica Drew (voice)
Elizabeth Perkins May / Quippy Spider-Person (voice)
Amandla Stenberg Margo Kess: Spider-Byte (voice)
Karan Soni Pavitr Prabhakar (voice)
Brian Tyree Henry Jeff Morales (voice)
Lex Lang Additional Voices (voice)
Luna Lauren Vélez Rio Morales (voice)
Shameik Moore Miles Morales (voice)
Cliff Robertson Uncle Ben (archive footage) (uncredited)
Phil Lord Producer, Additional Voices (voice), Writer
Ayo Edebiri Glory (voice)
Taran Killam Web-Slinger (voice)
Amy Pascal Producer
A$AP Rocky Songs
Yuri Lowenthal Insomniac Spider-Man (voice)
Peggy Lu Mrs. Chen (uncredited)
Jharrel Jerome Miles G. Morales (voice)
Oscar Camacho Additional Voices (voice)
Jorma Taccone Adriano Tumino / '67 (voice)
Peter David Thanks
Becky G Songs
Ismail Bashey Additional Voices (voice)
Michelle Ruff Additional Voices (voice)
Joaquim Dos Santos Director
Sofia Barclay Malala Windsor: Spider-UK (voice)
Josh Keaton Spectacular Spider-Man (voice)
Kimiko Glenn Peni Parker (voice) (uncredited)
2 Chainz Songs
Rodney Rothman Executive Producer
Peter Sohn Ganke (voice)
Ziggy Marley Lenny (voice)
Pierre Perifel Animation
David Callaham Writer
Daniel Pemberton Original Music Composer
Hasan Minhaj Thanks
Arthur Ortiz Additional Voices (voice)
Bob Persichetti Executive Producer
Chrystee Pharris Additional Voices (voice)
Avi Arad Producer
Jacqueline Pinol Additional Voices (voice)
Christopher Miller Producer, Additional Voices (voice), Writer
Melissa Sturm Mary Jane (voice)
Metro Boomin Songs, Metro Spider-Man (voice), Executive Music Producer
Kemp Powers Director
Justin K. Thompson Director
Natalia Castellanos Additional Voices (voice)
Andrew Morgado Additional Voices (voice)
Mike Rianda Production Consultant, Additional Voices (voice)
Natasha Leonnet Digital Colorist
James Blake Songs
Michael Semanick Sound Re-Recording Mixer
Luisa Leschin Additional Voices (voice)
Will Digby Sound Effects Editor
Al Rodrigo Additional Voices (voice)